Figure 2 | Scientific Reports

Figure 2

From: Expression of human solute carrier family transporters in skin: possible contributor to drug-induced skin disorders

Figure 2

Functional analysis of SLC transporters in human skin cells.

(A), Total RNA samples of human skin, liver, small intestine and HaCaT cells were analyzed by RT-PCR using primers specific for PCFT and RFC. SI, small intestine; M, 100 base pair marker. Full-length gels are shown in the supplementary information. (B), Quantitative RT-PCR analysis of PCFT in human skin, liver, small intestine and HaCaT cells was conducted. Expression was normalized with the expression of GAPDH. (C), Uptake of FA into HaCaT cells was determined in the absence or presence of PCFT inhibitors. Column is the mean ± S.D. of three independent determinations. *, P < 0.05; **, P < 0.01, compared with the transport activity in the control.

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