Figure 5
The relative mRNA and protein expressions of HIF-1α and VEGF in the retina postoperatively.
No significant variations were found between the HIF-1α mRNA and protein in the silicone oil and FCVB + silicone oil groups at 30, 90 and 180 days postoperatively compared with the contralateral eyes (Fig. 5 a, b, c) (mRNA: p = 0.209, p = 0.853 and p = 0.797 in the silicone oil group, respectively; p = 0.147, p = 0.966 and p = 0.543 in the FCVB + silicone oil group, respectively. Protein: p = 0.163, p = 0.684 and p = 0.820 in the silicone oil group, respectively; p = 0.169, p = 0.678 and p = 0.719 in the FCVB group, respectively) and no significant differences were shown in the VEGF mRNA and protein in both treated groups at 30, 90 and 180 days postoperatively compared with in the contralateral eyes (d, e) (mRNA: p = 0.685, p = 0.350 and p = 0.567 in the silicone oil group, respectively; p = 0.984, p = 0.135 and p = 0.516 in the FCVB + silicone oil group, respectively. Protein: p = 0.745, p = 0.437 and p = 0.891 in the silicone oil group, respectively; p = 0.564, p = 0.335 and p = 0.744 in the FCVB + silicone oil group, respectively). The statistic data of each treated group is the ratio of HIF-1α or VEGF levels in the right (treated) eye to that in the left (contralateral) eye (n = 5 per group). Error bars represent the SEM.
