Figure 1
From: Omics-based interpretation of synergism in a soil-derived cellulose-degrading microbial community
Schematic representation of cellulosomal subunits identified in the F1RT consortium.
(A) Dockerin catalog and modular architecture of the various scaffoldins identified in the F1RT metagenome. Putative scaffoldins were affiliated to the draft genome bin FC2. Note that this is a partial genome bins, meaning that some proteins are incomplete and that we only have access to a subset of cellulosomal proteins. Domain architecture for all cellulosomal GH domains is summarized in Supplementary Table 10. Acronyms: GH, glycoside hydrolase; CBM, carbohydrate-binding module; Cu, copper; aa, amino acid. (B) Hypothetical example of a polycellulosome assembly that was predicted via metaproteomic analysis of soluble protein-protein complexes produced by the F1RT consortium (see Supplementary Fig. 14, Supplementary Table 21, “Band 10”). The shaded structure (corresponding to GL0023450) was not detected in the proteomics experiment. However, comparisons of ORF GL0026964 (lacking the 3′end), with the vanguard CipA scaffoldin of C.themocellum would indicate that it is likely that this protein encodes the necessary type-II dockerin that is found in GL0023450 (lacking the 5′end); see text.
