Table 2 FAH knockout mice

From: Efficient genetic manipulation of the NOD-Rag1-/-IL2RgammaC-null mouse by combining in vitro fertilization and CRISPR/Cas9 technology

     

Mutant Alleles per Mouse/Total Mice Tested

Gene

gRNA

Cas9

Transferred Embryos (Recipients/pregnancies)

Newborns (dead)

2 indel

1 indel

2KI

1KI

1indel +1 KI

0

FAH

gRNA3

WT Nuclease

211** (8/4)

11 (2)

3

1

1

2

2

0

 

gRNA1 + gRNA3

D10A Mut Nickase

147 (6/2)

11 (1)

3

0

0

0

0

7

  1. **201 injected embryos were transferred. One recipient received 11 injected and 10 non-injected embryos. This recipient did not produce any births.
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