Table 1 Summary of the electrophysiological properties of layer V pyramidal neurons

From: Extending the viability of acute brain slices

 

Control < 4 hrs (n = 10)

1–3 hrs (n = 12)

5–8 hrs (n = 7)

15–24 hrs (n = 9)

26–41 hrs (n = 9)

Resting membrane potential (mV)

−63 ± 2

−65 ± 2

−64 ± 1

−64 ± 1

−63 ± 1

Input resistance (MΩ)

176 ± 29

165 ± 18

163 ± 27

179 ± 21

145 ± 19

Membrane time constant (ms)

25 ± 4

28 ± 3

24 ± 7

25 ± 2

23 ± 3

Resonance frequency (Hz)

2 ± 0.3

2 ± 0.2

1.7 ± 0.3

1.5 ± 0.3

1.6 ± 0.3

First spike Amplitude (mV)

97 ± 5

97 ± 2

94 ± 4

93 ± 3

92 ± 3

Half-width spike amplitude (ms)

2.4 ± 0.2

2.5 ± 0.2

2.3 ± 0.1

2.6 ± 0.2

2.2 ± 0.1

mEPSC's frequency (Hz)

2.2 ± 0.3

1.75 ± 0.3

2.3 ± 0.4

1.5 ± 0.2

2.2 ± 0.3

mEPSC's amplitude (pA)

−7.3 ± 0.5

−6.4 ± 0.5

−7.8 ± 0.5

−5.7 ± 0.4

−8 ± 0.7

  1. Basic electrophysiological parameters were monitored during different incubation time in the Braincubator. Values are means ± S.E.M. No significant differences were detected between groups (one-way ANOVA). The first group on the left describes the same electrophysiological basic parameters recorded from neurons incubated under control conditions (<3 hours) for comparison.
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