Figure 1
Rest/wake behavioural assay in larval zebrafish.
(A) Crude herbs were prepared to obtain the extracting solution. Larvae (4 dpf) were then pipetted into each well of a 96-well plate (~360 μL) with different dilutions of TCM prescriptions at 9:00 AM. Zebrafish were observed beginning at 9:00 PM at 4 dpf. (B) As shown in this figure, the concentrations used were 10, 50, 100, 200, 500, 800 and 1000 μg/mL. Twelve larvae were prepared for each group. Parallel experiments were performed at least three times for each prescription. (C) Locomotor activity of a representative larva. Five parameters, including the total amount of rest, the number of rest bouts, the length of the rest bouts, the total activity and the waking activity, were analysed in detail. Each value represents the average of ~30 larvae and these parameters then generated the behavioural fingerprint of each prescription. (D) ESI-MS spectra in the positive ion voltage mode of the 1st, 2nd, 3rd and 5th prescriptions. Some of the constituents are labelled in the spectra.
