Figure 3
From: Differential requirement of GRP94 and GRP78 in mammary gland development
Morphology and proliferation of c94f/f mammary epithelium.
(a) H&E staining of 94f/f and c94f/f mammary glands from 1.5, 4, 7 and 11 month old virgin mice and 5 month old lactating mice on day 7 after parturition. Arrows denote TEBs. Scale bars show 200 μm and are applicable to all sections. (b) IHC staining of cell proliferative marker PCNA (brown nuclei) in mammary glands from 94f/f and c94f/f mice at 1.5 and 4 months. Scale bars show 100 μm and are applicable to all sections. Arrow heads point to PCNA positive cells. (c) Quantitation of percentage of PCNA positive epithelial cells in panel b. For each genotype, n varies from 2 to 4. PMSG was injected two days before euthanasia of 4, 7 and 11 month old mice.
