Figure 6

Morphology of c78^f/f mammary epithelium.

(a) H&E staining of 78^f/f and c78^f/f mammary glands from 1, 2, 4 and 5.5 month old virgin mice and 2.5 month old lactating mice on day 7 after parturition. Arrows denote TEBs. Scale bars show 200 μm and are applicable to all sections. (b) IHC staining of E-cadherin and α-smooth muscle actin (α-SMA) on serial sections from mammary glands of 1 month old 78^f/f and c78^f/f mice, with corresponding H&E staining. Brown color depicts E-cadherin or α-SMA staining. Arrows denote positive cells. Scale bars show 50 μm and are applicable to all sections. Negative controls for staining were shown in Supplementary Fig. 4e, f. (c) Quantitation of numbers of ducts from 78^f/f and c78^f/f mammary glands at 2, 4 and 5.5 months. For each genotype, n varies from 3 to 4.