Figure 1 | Scientific Reports

Figure 1

From: Generation of the SCN1A epilepsy mutation in hiPS cells using the TALEN technique

Figure 1

Schematic overview of the targeting strategy for the A5768G mutation The arrows with F0/R0 and F2′/R1′ indicate the primers used to test gene targeting.

“Nor” indicates the initial normal iPSCs. The donor plasmids contained Neo, the G418-resistance gene for drug screening. “+Nor” indicates the normal iPSCs after gene insertion with the Neo cassette. “Artificial patient” indicates the cells obtained after gene targeting and Cre-mediated excision of the drug cassette.

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