Figure 4
From: Generation of the SCN1A epilepsy mutation in hiPS cells using the TALEN technique
Characterization of differentiated neurons Immunofluorescence staining of the neuron-specific markers β-tubulin (red) and microtubule-associated protein 2 (green) in the neurons differentiated from the “Nor” (a/b/c) and the “AP” (d/e/f) cells. The scale is 100 μm. (g) RT-PCR analysis of the neurons indicated that gene manipulation did not disrupt expression of the Nav1.1 channel. “NC” indicates the negative control iPSCs. “Beta-actin” was used as the positive control (PC). “Nor” indicates the neurons differentiated from normal iPSCs and “AP” indicates the “artificial patient” neurons obtained after gene manipulation. The full-length gel is shown in Supplemental Figure S5.
