Figure 6
From: Generation of the SCN1A epilepsy mutation in hiPS cells using the TALEN technique
Electrophysiological characteristics of neurons differentiated from “artificial patient” iPSCs (a) shows a whole-cell neuronal recording in which spontaneous miniature excitatory post-synaptic currents (PSCs) were detected. (b) shows spontaneous action potentials (APs) without current injection. (c) indicates the evoked action potentials recorded after injection of step currents (−20 to 50 pA). (d) shows the voltage-gated sodium currents, indicated by the arrow, recorded following depolarizing voltage steps (−80 to 80 mV). (e) indicates the voltage-gated potassium currents evoked following depolarizing voltage steps (−80 to 80 mV).
