Figure 3
Knockdown of ME2 inhibits AKT activity in A549 cells.
(a), Basal AKT, PTEN and AMPK levels and activation in stable ME2 knockdown cells. The full-length blots are presented in Supplemental Figure S11. (b), Quantitatively analysis p-Akt in “a” by densitometry assay. (c), Inducible shRNA induces ME2 knockdown and inhibits basal AKT activity in A549 cells. The full-length blots are presented in Supplemental Figure S12. (d). Quantitatively analysis p-Akt in “c” by densitometry assay. (e), A549 cell with or without ME2 knockdown were starved for 24 h and stimulated with 100 ng/ml EGF. The full-length blots are presented in Supplemental Figure S13. (f). Quantitatively analysis p-Akt in “e” by densitometry assay. (g), Overexpression of human ME2 in HEK293 cells enhances AKT activation and decreases PTEN. For this figure, expression levels of ME2, p-AKT473, p-AKT308, AKT, p-PDK1, PDK1, p-ERK1/2, ERK1/2, p-PTEN, PTEN, p-AMPK, AMPK and β-tubulin were determined by Western blotting. The full-length blots are presented in Supplemental Figure S14. (h). Quantitatively analysis p-Akt in “g” by densitometry assay.
