Figure 5
NAC and GSH rescue exogenous DMM induced phenotype in A549 lung cancers.
(a), Accumulation of mitochondrially generated superoxide in A549 cells as detected by MitoSOX (Top: MitoSOX red; Bottom: Phase contrast). Data are representative of two independent experiments. (b), NAC and GSH inhibit the ROS induced by exogenous DMM in A549 cells. ROS in A549 cells detected by flow cytometry using CM-H2DCFDA. Each histogram is representative of three experiments. Left: A549 cells were treated with 10 mM DMM with or without 20 mM NAC or 20 mM GSH. Right: A549 cells were treated with 20 mM DMM with or without 20 mM NAC or 20 mM GSH. (c), NAC or GSH inhibits 10 mM DMM induced cell death. (d), NAC or GSH inhibits DMM induced cell differentiation and rescue the AKT inhibition. The full-length blots are presented in Supplemental Figure S16. (e), Quantitatively analysis p-Akt in “d” by densitometry assay.
