Figure 6
Stable knockdown of endogenous ME2 levels in A549 cells suppresses tumor formation.
(a), A549 cells transfected with ME2 of control shRNA were injected in nude mice separately. Tumor-bearing mice were sacrificed after 6 weeks and the tumors were dissected and weighed. (b), ME2 expression levels from tumor samples (a) were analyzed by Western blotting. The full-length blots are presented in Supplemental Figure S17. (c), A549 cells containing DOX inducible-ME2 specific shRNA or control shRNA cells were subcutaneously implanted into female athymic nude mice. 10 days later, Dox (2 mg/ml) were fed and tumor-bearing mice were sacrificed after 6 weeks and the tumors volume were measured. The tumor volume was calculate using the formula: V = 0.4AB2 (A: Long diameter; B: short diameter). (d), ME2 expression levels from tumor samples (d) were analyzed by Western blotting. The full-length blots are presented in Supplemental Figure S18. (e), Depletion of ME2 renders cells more sensitive to cisplatin treatment. Cells in experiments without cisplatin treatment were normalized to 1 and the cells treated with cisplatin were compared to its control. (f), Stable knockdown of ME2 combined with cisplatin treatment in A549 cells synergistically inhibits tumor size in nude mice. ME2 deficient cells from ME2 specific shRNA or control pLKO cells were subcutaneously implanted into female athymic nude mice. Tumor size was measured and calculated. (g), Depletion of ME2 inhibits mucin-1 and vimentin expression and increase differentiation marker E-cadherin and ZO-1 expression in vivo.
