Figure 4

Anti-dengue effect of continuous treatment with baicalin in Vero cells.

Foci forming unit reduction assay (FFURA) was used to evaluate the in vitro antiviral activity daily up to three days post infection (A). Anti-dengue activity of 50 μg/ml of baicalin (C); or with 25 μg/ml of baicalin (D) or without baicalin (E) using DENV foci immunostaining method on the day three post infection were shown. The respective DENV-2 RNA copy number was quantified using qRT-PCR (B). DENV-2 in vitro replication is inhibited more than 90% using 50 μg/ml of baicalin which is lower than MNTD. The percentages of foci reduction (% RF) and RNA copy number reduction were obtained by comparing against untreated controls maintained in parallel. Data from triplicate assays for three independent experiments were plotted using Graph Pad Prism Version 5 (Graph Pad Software Inc., San Diego, CA.).