Figure 1 | Scientific Reports

Figure 1

From: An activator of mTOR inhibits oxLDL-induced autophagy and apoptosis in vascular endothelial cells and restricts atherosclerosis in apolipoprotein E-/- mice

Figure 1

Effect of 3BDO on mammalian target of rapamycin (mTOR) activity in oxidized low-density-lipoprotein (oxLDL)-treated human umbilical vein endothelial cells (HUVECs).

(a), Western blot analysis of mTOR, phosphorylated mTOR (p-mTOR, Ser2448), p70S6K, phosphorylated p70S6K (p-p70S6K, Thr389), 4EBP1, phosphorylated 4EBP1(p-4EBP1, Thr37/46) in HUVECs treated with native LDL (nLDL), 50 μg/ml; oxLDL, 50 μg/ml; 3BDO-L, 60 μM; and 3BDO-H, 120 μM, for 12 h (cropped, full-length blots are in Supplementary Figure S6). (b), Densitometry results of ratio of p-mTOR to total mTOR, p-p70S6K to total p70S6K and p-4EBP1 to total 4EBP1. nLDL control data were set to 1. Data are mean ± SEM, n = 3.

Back to article page