Figure 7 | Scientific Reports

Figure 7

From: An activator of mTOR inhibits oxLDL-induced autophagy and apoptosis in vascular endothelial cells and restricts atherosclerosis in apolipoprotein E-/- mice

Figure 7

Effect of 3BDO on inflammatory response in oxLDL-treated HUVECs and apoE-/- mice.

(a) and (b), Western blot analysis of the effect of 3BDO on intracellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) level (full-length blots are in Supplementary Figure S9 and S10), with oxLDL, 50 μg/ml; 3BDO-L, 60 μM; 3BDO-H, 120 μM for 12 h. Protein levels were normalized to that of β-actin. Data are mean ± SEM. n = 3. (c) and (d), ELISA of the secretion of interleukin 6 (IL-6) and IL-8 from HUVECs with 3BDO treatment. oxLDL, 50 μg/ml; 3BDO-L, 60 μM; 3BDO-H, 120 μM for 12 h. Data are mean ± SEM. n = 3. (e) and (f), ELISA of the secretion of interleukin 6 (IL-6) and IL-8 in serum of apoE-/- mice. Data are mean ± SEM. n = 6.

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