Figure 4
Premature egg melanisation phenotype mediated by tyramine.
(a) Ovary dissection of PBS (control) and tyramine-injected females 3 days post-bloodmeal. (b) Representative examples of eggs dissected from female ovaries ~24 h after aminergic compound injection. (c) Mean ± SEM melanisation ratio of egg batches dissected from ovaries of 31–35 injected females from 3 repeats. (d) Mean ± SEM number of eggs laid by females following injection of tyramine alone or in combination with other compounds (N = 10 per experiment, minimum of 3 experiments, Student's t-test, in red p<0.05). (e) Proportion of females that survived 24 h post-injection with compounds (N = 10, minimum of 3 repeats, Student's t-test, in red p< 0.05). (f) Mean ± SEM melanisation ratio of egg batches laid by injected females (N = 10 per experiment, 3 experiments, Student's t-test, in red p< 0.05).
