Figure 5
From: Determination of Arabidopsis thaliana telomere length by PCR
Relative T/S ratios determined for the Arabidopsis wild type strain and for its ku70 and ddm1 mutant derivatives.
A box plot representation is shown. For each strain, MMQPCR reactions were performed using eight different amounts of bulk genomic DNA per PCR reaction, ranging from 20 ng to 0.04 ng. Six different technical repetitions were performed for each DNA amount. Therefore, 48 different T/S ratios were determined for every strain (corresponding to 8 DNA amounts and 6 technical repetitions). All the T/S ratios were normalized by dividing them between by the average T/S value obtained for all the wild type DNA amounts and technical repetitions. The resulting T/S values were used to perform the box plot representation. For statistical analyses, all the T/S values corresponding to each strain were pooled together. Then, the significance of differences between the wild type strain and each of the mutants were determined using the Student's two-tailed t-test (p < 0.001 for both comparisons).
