Figure 7
From: Determination of Arabidopsis thaliana telomere length by PCR
DNA methylation mutants have shorter telomeres than the wild type strain.
(a) Box plot representation of relative T/S ratios determined for wild type plants (WT1 and WT2) and for a ddm1 mutant (ddm1) using bulk genomic DNA. The T/S ratios of individual plants segregated from a heterozygous met1-7 mutant are also shown. The progeny of the met1-7 heterozygous includes three wild type plants (wt1, wt2 and wt3) and one homozygous mutant plant (met1-7). MMQPCR reactions were performed using about 2 ng of DNA per reaction. At least five different technical repetitions were performed for each bulk or individual plant DNA sample. All the T/S ratios were normalized by dividing them between the average T/S value obtained for the WT1 and WT2 DNA samples. The resulting T/S values were used to perform the box plot representation. Statistical significances of differences between the T/S values obtained with the DNA samples of WT1 or WT2 and the other DNA samples were determined using the Student's two-tailed t-test. The highest p values obtained for each couple of comparisons (with WT1 or WT2) are indicated in the panel. (b) Box plot representation of relative T/S ratios determined for wild type plants (WT1 and WT2) using bulk genomic DNA and for individual drm1-2, drm2-2 and cmt3-11 triple mutant plants (ddc1 to ddc5). MMQPCR and statistics analyses were performed as indicated in (a).
