Figure 2

Growth arrest of human GBM via CG500354-mediated p53 overexpression.

(A) Cell cycle analysis shows that the percent of cells in G1 phase was increased after CG500354 treatment. (B–C) Quantitative RT-PCR analysis shows that the cell cycle regulators cyclin A1, B1, D1 and D3 were significantly down-regulated and that the Cdks p21 and p27 were significantly up-regulated in the presence of CG500354. (D) Western blot analysis shows that p53 expression was increased by treating the cells with 1 μM or 3 μM CG500354. The full-length blots are included in the supplementary information (Suppl. Fig. S5). (E–F) GBM-derived cells treated with CG500354 were stained with a p53 antibody. The number of p53-expressing cells (green) were significantly increased after CG500354 treatment at both concentrations (1 μM and 3 μM). Nuclei (blue) were counterstained with DAPI. Scale bar = 0.5 mm.