Figure 1
From: Similar morphological and molecular signatures shared by female and male germline stem cells
Confirmation that FGSCs possess germline stem cell properties similar to SSCs.
(A) Freshly isolated FGSCs and SSCs have a round shape with large, bright, spherical nuclei; there is an obvious boundary between the cytoplasm and the nucleus. Isolated FGSCs and SSCs form grape-like clusters after 2 days of culture. Scale bar = 10 μm. (B) RT-PCR analysis of germ cell markers. M, 100 bp DNA marker; lane 1, freshly isolated FGSC; lane 2, 2-day cultured FGSC; lane 3, freshly isolated SSC; lane 4, 2-day cultured SSC; lane 5, no template control. (C) Immunofluorescence analysis of MVH, Oct4 as well as alkaline phosphatase (AP) was used for characterization and DPAI was used to stain the nucleus. Similar to SSCs, FGSCs are positive for MVH and Oct4. FGSCs are weakly stained by the AP assay, similar to SSCs. Scale bar = 10 μm.
