Figure 6

a) MTT assay of HeLa cells with free MB molecules and MB-loaded Au@polymer NPs at 24 h. b) Bright-field image of the HeLa cells with MB-loaded Au@polymer NPs and two SERS mapping images (based on the 1623 cm−1 vibration of MB) collected for the rectangle shown in the bright field of a HeLa cell. N indicates the nucleus. c) Fluorescence images of DCFH-DA staining in HeLa cells incubated with MB-loaded Au@polymer NPs and free MB for 4 h. The sample-treated cells were exposed to LED light at 660 nm (30 mW/cm2) for 4 min before imaging detection. d) Fluorescence intensity of MB-loaded Au@polymer NPs and free MB in HeLa cells.