Figure 2
Different temperature sensitivities of TR-FRET signals of HTT-exon1 proteins.
(a) The Western-blot of HTT-exon1 Q72 and Q25, using HTT N-terminal antibody 2B7. (b) The Western-blot using the polyQ antibody MW1 for the same lysates used in (a). (c) 2B7/MW1 TR-FRET signals of the lysates from the transfected cells at 4°C (4C) and room temperature (RT, 22°C). Data plotted as average ± s.e.m. (n = 3). (d) 4C9/MW1 TR-FRET signals of the lysates from the transfected cells at 4°C (4C), room temperature (RT, 22°C) and 37°C (37C). Data plotted as average ± s.e.m. (n = 3). (e) Upper panels: 2B7/MW1 TR-FRET signals of the Q72 (left) and Q25 (right) HTT-exon1 transfected samples at different concentrations (μg/well). Note that the signal plateau has been reached. The lower panel: the ratio between 4C and RT for Q72 and Q25 samples at different concentrations (μg/well). Data plotted as average ± s.e.m. (n = 4).
