Figure 5
Higher temperature sensitivity of the Q25 TR-FRET signal is due to structural or spatial changes of HTT polyQ.
(a) Western-blots of HEK293T cells transfected with different HTT-exon1 Q25 and Q72 constructs with point mutations as indicated. The last lane on each blot is from non-transfected HEK293T lysate. (b) Temperature sensitivity of the 4C9/MW1 TR-FRET signals. All signals are normalized to the signal at room temperature (RT), n = 3. Q25 has higher temperature sensitivity for all the HTT-exon1 constructs with or without point mutations. (c) Temperature sensitivity of the 2B7/3B5H10 TR-FRET signals for Q25 and Q72. All signals are normalized to the signal at room temperature (RT), n = 6. Between 4°C and RT, Q25 has higher temperature sensitivity than Q72.
