Table 2 Validation of in vivo differential expression from transcriptional profile of Acidovorax avenae subsp. avenae strain RS-1 by the changes in pathogenicity of T6SS gene mutants constructed in this study

From: Transcriptome analysis of Acidovorax avenae subsp. avenae cultivated in vivo and co-culture with Burkholderia seminalis

  

NGEVa of RNA-Seq

Validation by mutation

Locus Tag

T6SS components

In vitro

In vivo

Gene

Pathogenicity

Acav_1267

ClpB

5.9

2

ΔclpB

loss

Acav_1504

Hcp

123.6

24.85

Δhcp

loss

Acav_1905

VgrG-8

11.95

5

ΔvgrG

loss

Acav_1512

IcmF

6.4

1.8

ΔicmF

loss

Acav_1519

DUF879

5.85

2.6

ΔdotU

loss

Acav_1511

DotU/OmpA/MotB

11.05

4.4

ΔompA

loss

Acav_2399

VgrG-3

4.05

0

ΔimpA

loss

Acav_1516

Duf877/EvpB

68.35

22.55

ΔevpB

decrease

Acav_0662

VgrG-2

13.7

0

ΔvgrG

loss

Acav_0298

VgrG-1

1.65

3.7

ΔvgrG

loss

Acav_1513

ImpM

4.85

1.9

ΔimpM

loss

Acav_4620

PppA

8.95

6.1

ΔpppA

Not loss

Acav_1509

Lip

12.65

3.55

Δlip

Not loss

  1. aNGEV: Normalized gene expression value. The detailed knockout of these T6SS genes and their pathogenic function will be published in another paper.
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