Figure 2

The loss of color from the host A. pulchella in 30 days.
A. pulchella was cultured in enriched medium (A–C) and in medium depleted nitrogen source for the indicated times (D–I). The morphological changes of sea anemone growth under N sufficient and limited conditions (A)(D)(G). The tentacles of its host sea anemone went bleaching during 30 days; enlarged portions labeled by solid rectangular boxes (B)(E)(H). Fluorescence microscopy was applied to detect the auto-fluorescence of Symbiodinium chlorophyll (C)(F)(I). The scale bars indicate 200 μm.