Figure 2
From: The K898E germline variant in the PP1-binding motif of BRCA1 causes defects in DNA Repair
Reconstitution of BRCA1 K898E is unable to reduce hypersensitivity to IR in Brca1-dysfunctional cells.
(a) WT and mutant BRCA1 proteins were expressed at similar levels in 780 cells. γ-tubulin was used as a loading control. Full length immunoblots are shown in Supplementary Information section (Supplementary Fig. S2c online). (b) Post-IR survival in WT and mutant BRCA1 expressing 780 cells measured by the colony formation assay. Results are presented as mean ± SEM of at least three independent experiments, with each experiment performed in triplicate. * P < 0.05, ** P < 0.01.
