Table 1 Environmental parameters, Shannon-Wiener index, dominant species' relative abundance and copy number of DNA and RNA in the Dongzhen Reservoir

From: Effects of water stratification and mixing on microbial community structure in a subtropical deep reservoir

Water layer

Mixing layer (n = 5)

Epilimnion (n = 6)

Metalimnion (n = 3)

Hypolimnion (n = 6)

Temperature (°C)

16.17 ± 0.01a

25.04 ± 1.22b

16.87 ± 0.37a

14.18 ± 0.22c

DO (mg l−1)

8.23 ± 0.07a

6.16 ± 1.41a

2.26 ± 2.04b

2.82 ± 1.07b

pH

7.46 ± 0.02a

7.75 ± 0.58a

6.64 ± 0.14ab

6.34 ± 0.10b

Chl-a (μg l−1)

3.24 ± 0.15a

2.39 ± 0.78a

0.73 ± 0.27b

0.82 ± 0.14b

TN (mg l−1)

3.68 ± 0.17a

2.86 ± 0.53a

3.14 ± 1.10a

4.38 ± 1.17a

NH4-N (mg l−1)

0.10 ± 0.02a

0.18 ± 0.04a

0.13 ± 0.11a

0.20 ± 0.08a

NOx-N (mg l−1)

1.42 ± 0.06a

1.15 ± 0.14a

1.28 ± 0.18a

1.34 ± 0.10a

TP (mg l−1)

0.042 ± 0.008a

0.038 ± 0.004ab

0.026 ± 0.007ab

0.025 ± 0.004b

PO4-P (mg l−1)

0.002 ± 0.001a

0.009 ± 0.004a

0.014 ± 0.011a

0.011 ± 0.005a

TN/TP mass ratio

100.70 ± 116.94ab

79.46 ± 16.66a

120.99 ± 37.32ab

165.62 ± 29.89b

Shannon-Wiener index

4.00 ± 0.06ab

3.77 ± 0.13a

4.16 ± 0.04b

4.18 ± 0.09b

Actinobacteria (%)

0.706 ± 0.012a

0.625 ± 0.017b

0.629 ± 0.015b

0.589 ± 0.020b

Proteobacteria (%)

0.139 ± 0.006a

0.183 ± 0.023a

0.204 ± 0.036a

0.216 ± 0.014a

Cyanobacteria (%)

0.075 ± 0.004a

0.045 ± 0.025ab

0.033 ± 0.020ab

0.052 ± 0.020b

Unknown bacteria (%)

0.038 ± 0.001a

0.039 ± 0.008a

0.052 ± 0.006ab

0.059 ± 0.006b

Copy number of DNA (l−1)

(1.14 ± 0.14) × 1010a

(7.92 ± 1.60) × 1010b

(1.06 ± 0.14) × 1011c

(4.45 ± 0.51) × 1010b

Copy number of RNA (l−1)

(6.58 ± 0.16) × 1011a

(3.89 ± 0.72) × 1011b

(2.80 ± 0.60) × 1011b

(8.36 ± 1.36) × 1011c

RNA/DNA ratio

6.31 ± 1.70a

10.59 ± 2.85a

3.01 ± 0.64a

23.17 ± 3.28b

  1. Analysis of variance (ANOVA) was used in combination with Scheffe's F multiple-comparison test to examine differences among the parameters of the sampling sites. Different lower case letters indicate significant differences among the sites.
  2. Data represent mean ± SE.