Figure 4
From: Chrysanthemum CmNAR2 interacts with CmNRT2 in the control of nitrate uptake
The interaction between CmNRT2 and CmNAR2 based on the DUAL membrane pairwise interaction assay.
Cells grown on media lacking (A) Ade, His, Leu and Trp, (B) Leu and Trp. (C) β-galactosidase assay of the interaction between CmNRT2 and CmNAR2. Yeast strain NMY51 carried each pairs of bait and prey plasmid (pBT3-C and pPR3-N are the control vectors with no cloned cDNA). The construct pair of CmNRT2 and CmNAR2 fusions was used as positive control. The construct pairs of CmNRT2 and CmNAR2 with empty vector, respectively, were used as negative controls.
