Figure 1

Tyk-2 deficiency induced mast cell differentiation and IL-9 in the lung of asthmatic mice.

(a). experimental design (b). Lung histology for mast cell detection with Toluidine Blue staining. (c). Total lung cells from naïve and OVA treated mice were incubated with antibodies against CD117 (c-kit), CD123 (IL-3Rα) and FcεRI and analyzed by flow cytometry (n = 2–4; p = 0.05). (d).–(f). Lung CD4⁺T cells from naïve (PBS) and OVA treated mice were isolated and incubated for 24 h with α-CD3 and α-CD28 antibodies. The supernatants were analyzed by ELISA for IL-3 (d), IL-9 (e) and IL-10 (f) ((d): n = 4; p = 0.002; p = 0.004; (e): n = 3–4; p = 0.037; p = 0.015; p = 0.024; (f): n = 3; p = 0.012, respectively) (g). Release of IL-10 by total lung cells ((g): n = 3; p = 0,049; p = 0,02) (h). and (i). Irf4 and Pu.1 mRNA expression in total lung ((h): n = 2–4; p = 0,001 p = 0,007; (i): n = 3–4).