Figure 4

Tyk-2^(−/−) naïve CD4⁺ T cells showed a defective Th17 cytokine production ex vivo.

Spleen CD4⁺CD62L⁺ T cells were isolated from naïve Tyk-2^(−/−) and wild-type mice and treated with antibodies against CD3 (2 μg/ml), CD28 (2 μg/ml), IL-4 (10 μg/ml) and IFNγ (10 μg/ml) while recombinant cytokines TGF-β (3 ng/ml), IL-6 (20 ng/ml), IL-23 (50 ng/ml) and IL-21 (80 ng/ml) were added as indicated. After five days the supernatant was collected and analyzed by ELISA for IL-17A ((a): n = 2, p = 0,004, p = 0,009, p = 0,0008, p = 0,0003, p = 0,006) IL-10 ((b): n = 2, p = 0,003) and IL-17F ((c): n = 2, p = 0,001, p = 0,004, p = 0,0003).