Figure 2
Functional complementation test of candidate genes.
T1 lines that have been PCR validated to harbor the gene were used in the bioassays. The BPH2-avirulent biotype was used in these experiments. (a) The survival number of brown planthopper (BPH) on the three rice lines. The 1A5 line is the transgenic plant with the predicted gene on the DNA fragment 1A5 in the background of Taichung 65. The vector line is the transgenic plant with only vector fragment in the background of Taichung 65. BPH26-NIL is the near isogenic line with BPH26 in the genetic background of Taichung 65. In all, sixty last instar larvae that had been fasted for 24 h were released on each rice line for 5 days. (b) The amount of honeydew excreted by BPH females on the three rice lines. Values represent mean and SE (n = 10). Means labeled with the same letters are not significantly different by Tukey–Kramer test (P < 0.05). (c) The total duration of phloem ingestion on the three rice lines. The feeding behavior of BPH females was measured using an AC-EPG system for 10 h. Values represent mean and SE (n = 10). Means labeled with the same letters are not significantly different by Tukey–Kramer test (P < 0.05). (d) The total number of probes. The feeding behavior of BPH females was measured using an AC-EPG system for 10 h. Values represent mean and SE (n = 10). Means labeled with the same letters are not significantly different by Tukey–Kramer test (P < 0.05).
