Figure 5
From: A tool kit for rapid cloning and expression of recombinant antibodies
Antibody cloning by PIPE enables parallel characterization of different antibody isotypes.
Utilizing the PIPE cloning method seven different human isotype expression vectors were seamlessly generated for production of antibodies in HEK 293-F cells. Purified grass pollen allergen Phl p 7 specific 102.1F10 isotypes were analyzed by SDS-PAGE under reducing conditions, alongside with Protein Ladder (10-250 kDa) (NEB) and visualized by Coomassie blue staining (HC, heavy chain, LC, light chain).
