Figure 2
From: MicroRNA-769-3p Down-regulates NDRG1 and Enhances Apoptosis in MCF-7 Cells During Reoxygenation
MiR-769-3p is down-regulated in hypoxia and up-regulated upon re-oxygenation.
(A) Relative expression levels of miR-769-3p in normoxia (O2), hypoxia (N2) and reoxygenation (Re-O2) using quantitative RT-PCR. Breast cancer MCF-7 cells were cultured under normoxia, hypoxia for 24 h, or 24 h of hypoxia followed by 24 h of reoxygenation. The relative expression level of each condition was compared to hypoxia; 18s rRNA was used as an internal control. *: P < 0.05. (B) Relative expression levels of precursor mir-769-3p at different oxygen levels using quantitative RT-PCR.
