Figure 1
From: Transfer of gut microbiota from lean and obese mice to antibiotic-treated mice
Gut microbiota analysis by denaturing gradient gel electrophoresis (DGGE).
(a) Feces samples were collected from ampicillin-treated B6 recipient mice just before inoculation. The picture shows a segment of a gel illustrating DGGE profiles of three weeks old ampicillin-treated mice before they were randomized to receive microbiota inoculation from donor mice. Each lane represents the gut microbiota profile of one mouse. (b) Principal Component Analysis (PCA) plot of DGGE profiles from feces samples collected from recipient mice one week after inoculation at three weeks of age with either C57BL/6 (green balls n = 12; donor: light green ball n = 1) or B6.-Lepob (orange balls n = 13; donor: pink ball n = 1) microbiota. (c) PCA plot of DGGE profiles from feces samples collected from recipient mice six weeks after inoculation at three weeks of age with either C57BL/6 (green balls n = 12; donor: light green ball n = 1) or B6.-Lepob orange balls n = 13; donor: pink ball n = 1) microbiota. (d) PCA plot of DGGE profiles from feces samples collected from recipient mice one week after inoculation at eight weeks of age with either BALB/c (dark blue balls n = 14; donor: light blue ball n = 1), B6.-Lepob (orange balls n = 15; donor: pink ball n = 1) microbiota, or PBS (yellow balls n = 14). (e) PCA plot of DGGE profiles from feces samples collected from recipient mice six weeks after inoculation at eight weeks of age with either BALB/c (dark blue balls n = 14; donor: light blue ball n = 1), B6.-Lepob (orange balls n = 13; donor: pink ball n = 1) microbiota, or PBS (yellow balls n = 15). (f–h) Scatterplots of PC1 (x-component), PC2 (y-component) and PC3 (z-component) respectively from DGGE profiles of feces samples shown in D. (i–k) Scatterplots of PC1 (x-component), PC2 (y-component) and PC3 (z-component) respectively from DGGE profiles of feces samples shown in E. (l) The picture shows a segment of a gel illustrating DGGE profiles of mice one week after inoculation with BALB/c microbiota (blue), B6.-Lepob microbiota (orange), or PBS (yellow). The red circles mark examples of differences in appearance of bands between the two inoculated groups. (m) The picture shows a segment of a gel illustrating DGGE profiles of mice six weeks after inoculation with BALB/c microbiota (blue), B6.-Lepob microbiota (orange), or PBS (yellow).
