Figure 3
From: Transfer of gut microbiota from lean and obese mice to antibiotic-treated mice
Microbiota transfer at weaning changes metabolic parameters in antibiotic-treated recipient mice.
(a) Weekly body weight (gram) of recipient mice after inoculation with either C57BL/6 (males n = 3; females n = 7) or B6.-Lepob microbiota (males n = 4; females n = 8) at three weeks of age. (b) Area under the curve (AUC) calculated from weight data in A. (c) AUC of weekly food consumption (gram) per mouse in average per cage in six weeks from inoculation in recipient mice inoculated with either C57BL/6 (males n = 2; females n = 4) or B6.-Lepob microbiota (males n = 4; females n = 3) at three weeks of age. (d) Weight (gram) of body fat pads (the sum of inguinal fat pads, retroperitoneal pad pads, epididymal fat pads or uterine fat pads in males and females respectively) in recipient mice six weeks after inoculation with either C57BL/6 (males n = 3; females n = 9) or B6.-Lepob microbiota (males n = 4; females n = 8) at three weeks of age. (e) HbA1c (glycated haemoglobin, %) concentration in the blood of recipient mice six weeks after inoculation with either C57BL/6 (n = 9, green bars) or B6.-Lepob (n = 11, orange bars) microbiota at three weeks of age. (f–h) Oral glucose tolerance test (OGTT) one, four and six weeks (respectively) after inoculation with either C57BL/6 (n = 12) or B6.-Lepob (n = 12) microbiota at three weeks of age. Tail blood glucose relative to baseline value (−15 min) is shown at given time points after oral gavage of glucose. * represents P < 0.05 difference in peak values. (i) Fasting blood insulin levels relative to insulin levels at inoculation at three weeks of age. Insulin levels are shown four and six weeks post inoculation (PI). * represents P < 0.05. Mean ± SEM.
