Figure 4
From: Spermatogonial stem cell enrichment using simple grafting of testis and in vitro cultivation
Flow-cytometric analysis and RT-PCR of cultured SSCs from adult mouse testis tissues 2 weeks after grafting into recipient nude mice.
(A) The percentage of Thy-1 and GFR-α1-positive cells in the non-graft group was only 7.33 and 3.93%, respectively. The percentage of Thy-1 and GFR-α1-positive cells in the graft (whole) group was 85.00 and 78.92%, respectively, which was significantly higher than that in the non-graft group. The percentage of Thy-1 and GFR-α1-positive cells in the graft (slice) group was 83.40 and 68.26%, respectively, which was also significantly higher than that in the non-graft. (B) Germ cell-specific mRNA expression patterns by real time RT-PCR. Gfr-α1, lin 28 and, ngn3 mRNA were used as undifferentiated spermatogonia marker genes. c-kit, piwil2 and stra8 were used for differentiating spermatogonia and spermatocyts. TH2B was used for spermatocytes. TP-1 mRNA was used as a spermatid marker gene, MVh was used for all germ cells and 18S ribosomal RNA was used as a control gene.
