Figure 1
From: A nucleolytic lupus autoantibody is toxic to BRCA2-deficient cancer cells
5C6 is a nucleolytic lupus autoantibody.
(A): 5C6 degrades single-stranded DNA in a time-dependent manner. Single-stranded M13mp18 circular DNA was incubated with buffer containing 2.5 μM 5C6 for 0–60 minutes, followed by visualization of DNA on an agarose gel. (B): The percentage of M13mp18 DNA remaining after incubation with 5C6 was quantified relative to untreated M13mp18 DNA. (C): 5C6 degrades single-stranded DNA in a dose-dependent manner. M13mp18 DNA was incubated with buffer containing 0–2.5 μM 5C6 for 10 minutes, followed by visualization on an agarose gel. (D): The percentage of M13mp18 DNA remaining after incubation with 5C6 as described in C was quantified relative to untreated M13mp18 DNA. (E): 5C6 degrades double-stranded DNA. pBluescript double-stranded plasmid DNA was incubated with buffer containing 6.6 µM 5C6 for 0–24 hours followed by visualization on an agarose gel. (F): The percentage of pBluescript plasmid DNA remaining after incubation with 5C6 as described in E was quantified relative to untreated pBluescript. C = circular conformation. L = linear conformation. N = nicked conformation. S = supercoiled conformation. Error bars: SEM.
