Figure 3
From: A nucleolytic lupus autoantibody is toxic to BRCA2-deficient cancer cells
5C6 has a differential impact on BRCA2-proficient and deficient DLD1 cells.
(A) and (B): BRCA2+ and BRCA2- DLD1 cells were treated with control media or media containing 10 µM 5C6 for 1 hour. Cells were then washed, fixed and probed for the presence of γH2AX with an Alexa-488 conjugated antibody. Light and immunofluorescence images are presented. Scale bar = 100 μm. (C): The percentage of γH2AX-positive BRCA2- cells after treatment with control or 5C6 was quantified. 5C6 increased the percentage of γH2AX-positive cells ~5-fold relative to control media. *p = 0.03 (n = 4). (D): 5C6 is toxic to BRCA2- DLD1 cells. BRCA2+ and BRCA2- DLD1 cells in subconfluent monolayers were treated with control media or media containing 10 µM 5C6 for 4 days. Cells were then harvested and counted using trypan blue. Percent growth inhibition relative to cells treated with control was determined. Percent growth inhibition is presented. 5C6 did not notably impact the relative percentage of viable BRCA2+ cells but significantly suppressed the growth of the BRCA2- cells. *p = 0.01 (n = 6). Error bars: SEM.
