Figure 2 | Scientific Reports

Figure 2

From: Introduction of correlative light and airSEMTM microscopy imaging for tissue research under ambient conditions

Figure 2

Integrative visualization of collagen type 1 fibrils by LM and airSEMTM complemented by TEM and conventional SEM images.

(a) The collagen fibrils were visualized by LM and a ROI was chosen for further inspection by airSEMTM. (b) High resolution airSEMTM image of the ROI. The beam energy is 30 keV. (c) TEM image of collagen fibril. Accelerating voltage is 120 keV. (d) Image of collagen fibril obtained by conventional SEM. The beam energy is 1.5 keV. Scale bar: (a) 50 μm. (b,c,d) 1 μm.

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