Figure 3 | Scientific Reports

Figure 3

From: Persistent prion infection disturbs the function of Oct-1, resulting in the down-regulation of murine interferon regulatory factor-3

Figure 3

Mutation analysis of murine IRF-3 promoter.

(a) Nucleotides sequences of the promoter region (nt −119 to −1) and the putative binding sites for the transcription factors are indicated on the sequence. (b) Nucleotide sequences of the original and mutated (M1 and M2) Oct-1 binding sites. (c) The promoter activity of N2a cells transiently transfected with pRL-null and pGL3 -119/-1 or pGL3 -119/-1 (M1). (d) The promoter activity of 3T3 cells transiently transfected with pRL-null and pGL3 -119/-1 or pGL3 -119/-1 (M1). (e) The promoter activity of N2a cells transiently transfected with pRL-null and pGL3 -119/-1 or pGL3 -119/-1 (M2). (f) The promoter activity of 3T3 cells transiently transfected with pRL-null and pGL3 -119/-1 or pGL3 -119/-1 (M2). (g) Nucleotide sequences of the original and mutated (M3) E2F1 binding sites. (h) The promoter activity of N2a cells transiently transfected with pRL-null and pGL3 -119/-1 or pGL3 -119/-1 (M3). (i) The promoter activity of 3T3 cells transiently transfected with pRL-null and pGL3 -119/-1 or pGL3 -119/-1 (M3). Results were normalized to the co-expressed renilla luciferase activity and the value of the original activity was set to 100%. Results represent the mean ± SD, n = 4. P values were determined by Student's-t test (***: P < 0.001; **: P < 0.01).

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