Figure 1
From: FAK and paxillin dynamics at focal adhesions in the protrusions of migrating cells
Dynamics of FAK and paxillin in a live EC transfected with GFP-FAK (green) and mCherry–paxillin (red).
(A) FAK and paxillin in the cell. The three boxed regions are cell front (B), center (C) and rear (D), with enlarged views shown in panels (B), (C) and (D), respectively. B–D show the results for FAK, paxillin and FAK + Paxillin at 0, 30 and 60 min. In these ratio photos, superimposition of GFP-FAK (green) and mCherry-paxillin (red) images appears as yellow. During the 60-min of cell migration, there are many more GFP-FAK clusters than mCherry-paxillin at cell front (B), but they are more similar in numbers at cell center (C) and rear (D). The corresponding Supplementary Movies are S4–6 for B, S7 for C and S8 D. Scale bars: A = 20 μm, B = 15 μm, C = 4.5 μm and D = 12 μm. (E) FI ratio images of FAK/paxillin in the same cell as 1A at 0 (left) and 60 min (right). The cell contour at 60 min is outlined in solid white, whereas the initial cell contour (0 min) is outlined with dashed white. Pseudo-color for the FAK/paxillin FI ratio value ranges from low (blue) to high (red) (ROIs in both panels are cyan for cell front, green for center and pink for rear) (Supplementary Movie S9). Scale bar = 18 μm. (F) Time courses (over 60-min) of FAK/paxillin FI ratio at the three ROIs as shown in Figs. 1E. FI ratios of FAK/paxillin at cell front, center and rear were obtained from eleven migrating cells, each with seven time points. The bar graphs represent mean ± s.e.m. (F1) ROIs moving with the cell migration. (F2) ROIs staying at fixed positions (0-time).
