Figure 2
From: FAK and paxillin dynamics at focal adhesions in the protrusions of migrating cells
Dual-color images showing the dynamic motion of FAK and paxillin at the protrusion front of a live EC transfected with GFP-FAK (green) and mCherry–paxillin (red).
(A) An intact cell image showing (from left to right) FAK (green) + Paxillin (red), FAK (green), paxillin (red) and FAK/Paxillin FI ratio (pseudo-color). In FAK+Paxillin, the superimposition of FAK and Paxillin yield yellow (Supplementary Movie S10). (B) Enlarged images from the boxed regions in A row. During the 60-min time course, there were more FAK clusters (arrows) than paxillin at the protrusions of cell front (Supplementary Movies S11–13). (C) FAK/paxillin FI ratio image, pseudo-color ranges from low (blue) to high (red). Enlarged images were from the boxed region in A-“FAK/Pax Ratio”. FAK-FAs are more apparent at the protrusion front than paxillin during 60-min time course (Supplementary Movies 14–15). Scale bars: A = 20 μm; B and C = 7 μm.
