Figure 3
From: FAK and paxillin dynamics at focal adhesions in the protrusions of migrating cells
Image analysis of dynamics of FAK and paxillin at FAs at the protrusion front of an EC transfected with GFP-FAK and mCherry-paxillin.
(A) Co-localization of FAK (green) and paxillin (red) yielded a yellow color. Boxed region was selected as regions of interest shown in B. (B) Identified FAs were labeled with numbers. The pairs of FAK (left) and paxillin (right) at the same FAs were selected for quantification analysis. (C) The time courses of normalized intensities (y-axis) of FAK (green) and paxillin (red) in four FAs (No. 4, 5, 16 and 20, arrowed and labeled in B) are plotted. (D) The values of the time correlation for all 22 FAs in the same cell (including the four FAs in C) are plotted as a function of time shift between FAK and paxillin at FAs. Also plotted is the mean curve for all 22 FAs in this cell. The results of time correlation analysis of this cell (22 individual FAs) yielded a time shift of 4.21 ± 0.87 min (mean ± s.e.m.). The value is significantly different from 0 (p < 0.001) indicates the time shift at dynamic FAs.
