Figure 5
From: FAK and paxillin dynamics at focal adhesions in the protrusions of migrating cells
Dual-color images show the dynamic motion of FAK and actin filaments in protrusion of cell front in live EC transfected with GFP-FAK and RFP-actin.
(A-left) Cell images show the superimposition (“FAK/Actin”; yellow) of FAK (green) and actin (red). Scale bar = 20 µm. (A-right) The enlarged images for actin and FAK show the boxed region in A-left. Scale bar = 8 µm. FAK clusters are associated with actin filaments (arrows). (B) The dynamics of FAK and actin filaments in the box region (A-left). The superimposition (FAK/Actin; yellow) of FAK (green) and actin (red) show FAK associated with actin filaments at the leading edge of the protrusion front during the 60-min time course (arrows) (Supplementary Movie 16). Scale bar = 12.5 µm.
