Figure 6
From: FAK and paxillin dynamics at focal adhesions in the protrusions of migrating cells
The immunostaining images of an EC stained with FAK, paxillin and actin filaments.
(A) The fluorescence photomicrographs show FAK immunostained with anti-FAK (left column), F-actin stained with rhodamine-phalloidin (middle column) and merging of FAK (green) and F-actin (red) images, with a yellow color for co-localization of FAK and actin filaments (right column). FAK and actin filaments are shown to be co-localized. FAK plaques are present at the points where stress fibers end and some FAK plaques are present at cell periphery (arrows). Actin filaments show staining weakly (up arrow). Scale bar = 6 μm. (B) Fluorescence photomicrographs show FAK (left column) and paxillin (middle column) immunfluorescence stained with anti-FAK and anti-paxillin antibodies, respectively. Merging of FAK (green) and paxillin (red) images (right column), with a yellow color for co-localization of FAK and paxillin. At the cell periphery, there are many more FAK plaques than paxillin plaques (arrows). Scale bar = 10 μm.
