Figure 1
From: NEK9-dependent proliferation of cancer cells lacking functional p53
Identification of NEK9 by miRNA target screen.
(A) Fold changes in expression (miR-22/NC) of 14 cell-cycle regulators, shown as a heat map. Red letters indicate potential miR-22 target genes identified by in silico analysis. (B) Relative expression levels of five mRNAs identified by this screen were quantitated using TaqMan qRT-PCR assays. GAPDH was used as an internal standard. (Lane 1: HCT116; Lane 2: SW480; Lane 3: HCT116 p53-/-). (C) Protein levels of candidate genes in the presence of miR-22 were determined by immunoblot analysis. A 70 kDa protein that stained with amido black was used as a loading control. The original full scan images are available in Supplementary Figure 7. (D) Cell proliferation assay. Cells were counted 72 h after transfection with specified siRNAs, indicated below the graph. Two siRNAs, designated 1 and 2, were used to knock down each gene. Error bars indicate standard deviation (SD, n = 3).
