Figure 5 | Scientific Reports

Figure 5

From: The Regulatory Effect of UL-16 Binding Protein-3 Expression on the Cytotoxicity of NK Cells in Cancer Patients

Figure 5

NKG2D reverses the sULBP3-induced downregulation of NK cell cytotoxicity.

K562 (A), 7721 (B), or CD133−SW620 (C) cells were co-cultured with normal NK cells, with or without ULBP3-Fc (1 ng/ml), NKG2D-Fc (5 ng/ml) and NKp44-Fc (5 ng/ml). The cytotoxicity of NK cells was measured by LDH release after co-culture for 6 h at the indicated E:T ratios. (D) Normal NK cells were incubated with the indicated concentrations of ULBP3-Fc for 6 h and NKG2D expression in NK cells was measured by FCM. A representative graph of NKG2D expression is shown. (E) Combined data of NKG2D expression are shown. The data are representative of results obtained from at least 3 independent experiments. The results are expressed as the mean ± SEM. *P < 0.05, **P < 0.01.

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