Figure 5
MiR-198 suppresses FUT8 expression and inhibits tumor growth and metastasis in nude mice.
(A,B) Overexpression of miR-198 inhibits growth of xenografts in nude mice. Stably transfected human CRC SW1116 cells (SW1116/miR-198 or SW1116/NC) were injected subcutaneously into the right forelimb axilla to establish a xenograft model. The sizes of in-situ and resected tumors arising from cells transfected with/without the miR-198 overexpression plasmid (at 21 days post-injection) are shown in panels (A) and (B), respectively. (C) Tumor growth curves in the miR-198 overexpression and control groups. MiR-198 overexpression significantly suppressed xenograft tumor growth (P < 0.001, t-test of tumor sizes). (D,E,F) qRT-PCR(D), Western blotting (E) and immunofluorescence (F) analyses show that overexpression of miR-198 dramatically decreases the FUT8 mRNA and protein levels in xenograft CRC tumors. Statistical analysis is based on three independent experiments which have been run under the same experimental conditions. Representative immunofluorescence images are shown and statistical results are indicated. (G,H) Gross and microscopic examinations (G) and occurrence (H) of liver metastasis in the miR-198 overexpression and control groups. Arrow and the letter M indicate metastasis.
